How many samples are there when test subjects come through different recruitment strategies? This depends on what you are looking for. If you used the different recruitment methods to specifically draw from different populations, you can also consider them distinct samples. In general, it mainly depends on your definition what a sample constitutes in a particular case. You can recruit random people as they walk through the door and decide the first 100 to be your Sample 1 for Study X and the second 100 to be your Sample 2 for Study Y, but you could also call all 200 of them to be just one sample if it suits your needs.